Rhodococcus: A promising genus of actinomycetes for the bioremediation of organic and inorganic contaminants.

Rhodococcus is a genus of actinomycetes that has been explored by the scientific community for different purposes, especially for bioremediation uses. However, the mechanisms governing Rhodococcus-mediated bioremediation processes are far from being fully elucidated. In this sense, this work aimed to compile the recent advances in the use of Rhodococcus for the bioremediation of organic and inorganic contaminants present in different environmental compartments. We reviewed the bioremediation capacity and mechanisms of Rhodococcus spp. in the treatment of polycyclic aromatic hydrocarbons, phenolic substances, emerging contaminants, heavy metals, and dyes given their human health risks and environmental concern. Different bioremediation techniques were discussed, including experimental conditions, treatment efficiencies, mechanisms, and degradation pathways. The use of Rhodococcus strains in the bioremediation of several compounds is a promising approach due to their features, primarily the presence of appropriate enzyme systems, which result in high decontamination efficiencies; but that vary according to experimental conditions. Besides, the genus Rhodococcus contains a small number of opportunistic species and pathogens, representing an advantage from the point of view of safety. Advances in analytical detection techniques and Molecular Biology have been collaborating to improve the understanding of the mechanisms and pathways involved in bioremediation processes. In the context of using Rhodococcus spp. as bioremediation agents, there is a need for more studies that 1) evaluate the role of these actinomycetes on a pilot and field scale; 2) use genetic engineering tools and consortia with other microorganisms to improve the bioremediation efficiency; and 3) isolate new Rhodococcus strains from environments with extreme and/or contaminated conditions aiming to explore their adaptive capabilities for bioremediation purposes.

Use of soil actinomycetes for pharmaceutical, food, agricultural, and environmental purposes.

In this article, we reviewed the international scientific production of the last years on actinomycetes isolated from soil aiming to report recent advances in using these microorganisms for different applications. The most promising genera, isolation conditions and procedures, pH, temperature, and NaCl tolerance of these bacteria were reported. Based on the content analysis of the articles, most studies have focused on the isolation and taxonomic description of new species of actinomycetes. Regarding the applications, the antimicrobial potential (antibacterial and antifungal) prevailed among the articles, followed by the production of enzymes (cellulases and chitinases, etc.), agricultural uses (plant growth promotion and phytopathogen control), bioremediation (organic and inorganic contaminants), among others. Furthermore, a wide range of growth capacity was verified, including temperatures from 4 to 60 °C (optimum: 28 °C), pH from 3 to 13 (optimum: 7), and NaCl tolerance up to 32% (optimum: 0-1%), which evidence a great tolerance for actinomycetes cultivation. Streptomyces was the genus with the highest incidence among the soil actinomycetes and the most exploited for different uses. Besides, the interest in isolating actinomycetes from soils in extreme environments (Antarctica and deserts, for example) is growing to explore the adaptive capacities of new strains and the secondary metabolites produced by these microorganisms for different industrial interests, especially for pharmaceutical, food, agricultural, and environmental purposes.

Synthesis, characterization and application of new adsorbent composites based on sol-gel/chitosan for the removal of soluble substance in water.

In this work, new adsorbent composites from the silica precursor tetraethyl orthosilicate (TEOS) and chitosan have been successfully synthesized, denominated 20%Chi, 30%Chi and 40%Chi. The composites presented enhanced chemical and physical characteristics, with emphasis on the high surface areas between 374.94 m2/g to 886.31 m2/g. The application of the composites in the model system (TY - Tartrazine yellow dye), presented adsorption capacities dependent on the amount of chitosan in the composite (40%Chi > 30%Chi > 20%Chi). However, from the experimental data of the constituent materials, 30%Chi provided the greatest increase in the adsorption capacity in the monolayer, with values of 36%. This demonstrates that the amount of chitosan in the compound alters the arrangement of adsorption sites. The 30%Chi composite presented life cycle superior to 10 reuse cycles.

Synthesis of glutaraldehyde-modified silica/chitosan composites for the removal of water-soluble diclofenac sodium.

Composite materials are effective adsorbents for the removal of various types of contaminants, such as pharmaceutical products. However, they require improvement to achieve a good adsorption capacity. This study presents the development of a promising adsorbent: silica/chitosan modified with different proportions of glutaraldehyde, which involves the D-glucosamine units from chitosan. The developed materials were evaluated for their ability to remove diclofenac sodium. The adsorption data showed that the diclofenac adsorption efficiency increased with increasing degree of glutaraldehyde crosslinking. The equilibrium and kinetic data were well fit by the Liu and Elovich models, respectively, and the maximum adsorption capacity was 237.8 mg/g. Therefore, it can be assumed that the process is predominantly chemical and exothermic, with a high affinity between the adsorbents and diclofenac sodium. The adsorption mechanisms were investigated to better understand the interactions, and the predominance of covalent bonds with the self-polymerized glutaraldehyde was verified.